The Development of an Amperometric Enzyme Biosensor Based on a Polyaniline-Multiwalled Carbon Nanocomposite for the Detection of a Chemotherapeutic Agent in Serum Samples from Patients

Polyaniline was electrochemically polymerized onto the platinum electrode modified with a mixture of Nafion and multiwalled carbon nanotube (PANI/MWCNT/Nf/Pt) to detect ammonium ion. The nanobiosensor (ASNase/PANI/MWCNT/Nf/Pt) then prepared by immobilizing L-asparaginase (L-ASNase) on PANI/MWCNT/Nf nanocomposite. used for rapid sensitive detection serum concentration anticancer agent L-asparagine (L-Asp) during chemotherapy. has dynamic ranges zero 180 ?M. sensitivity 0.829 ?A ?M?1 cm?2, response time less than 30 s. limit 140 nM L-Asp. Michaelis–Menten constant ( K m ) measured be 36.2 mM. successfully applied determination L-Asp in blood samples leukemia patients.